The problems concerning assay of anti-ATLA by immunofluorescence, enzyme-immuno assays, gelatin particle agglutination and western blot methods.
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چکیده
منابع مشابه
Detection of Antinuclear Antibody in Childhood Rheumatic Diseases by Immunofluorescence Assay and Enzyme Immuno Assay
Background: Anti-nuclear antibodies (ANAs) are specific antibodies directed against a variety of nuclear antigens. Indirect immunofluorecence (ANA-IFA) and Enzyme Immunoassay (ANA-EIA) are commonly used methods for ANA detection. The positivity of ANA-IFA using HEp-2 cell substrate is 90-100% in systemic rheumatic diseases. On the other hand positivity of ANA-EIA is found to be much lower in di...
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Screening of blood and blood products for human immunodeficiency virus (HIV) is routinely performed using the enzyme-linked immunosorbent assay (ELISA), and the results confirmed by western blot (WB). In an effort to identify alternative techniques, two locally prepared test kits were evaluated to determine the performance in comparison to commercial kits. These diagnostic kits were indirect im...
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ژورنال
عنوان ژورنال: Journal of the Japan Society of Blood Transfusion
سال: 1987
ISSN: 1883-8383,0546-1448
DOI: 10.3925/jjtc1958.33.7